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. 2017 Jun 27;8(40):67329–67343. doi: 10.18632/oncotarget.18622

Figure 5. ANCR attenuated TGF-β1 induced EMT and migration by decreased RUNX2 expression.

Figure 5

(A-B) RUNX2 mRNA and protein level was assessed by Real-time PCR and western blot assays in MCF10A-Vector and MCF10A-ANCR cells. (C) The morphological change of MCF10A-RUNX2 cells, Scale bars: 200 μm. (D) Western blots of the epithelial marker E-cadherin, and the mesenchymal markers (Fibronectin, Vimentin, α-SMA) in MCF10A-Vector and MCF10A-RUNX2 cells. (E-F) Invasion and migration assays in MCF10A-Vector and MCF10A-RUNX2 cells. (n=3;* P<0.05,** P<0.01,*** P<0.001).