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. 2017 Jun 27;8(40):67406–67421. doi: 10.18632/oncotarget.18684

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Copyright: © 2017 Schaefer and Behrends

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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The correct protein expression of the newly generated monoclonal stable K562 cell lines was analyzed by SDS-PAGE of cell extracts, followed by Western blot, detected with specific GFP and HO-1 antibodies. Actin was detected as loading control. (A) Controls-: Untransfected K562. GFP: Stable K562 cell line expressing GFP. (B) ER resident K562 GFP-HO-1 1-6. (C) Anchorless variant K562 GFP-HO-1-ΔC266 7-13. Left and right: ladder [kDa].