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. 2017 Jun 27;8(40):67406–67421. doi: 10.18632/oncotarget.18684

Figure 2. Analysis of the subcellular localization of GFP, GFP-HO-1 and GFP-HO-1-ΔC266 in the positively characterized stable K562 cell lines by confocal laser scanning microscopy.

Figure 2

(A) In the monoclonal stable K562 cell line expressing GFP, the protein is distributed in cytosol and nucleus. (B) In the monoclonal stable K562 cell lines 1-6 GFP-HO-1 is localized at the ER membrane. The nucleus is free from GFP-HO-1. (C) In the monoclonal stable K562 cell lines 7-13 GFP-HO-1-ΔC266 is distributed in cytosol and nucleus. Figure shows representative data from three pictures per sample measured on a CLSM at 37°C. Bar represents 20 μm.