Figure 4. Hemoglobin synthesis experiments in a K562 cell line expressing GFP.

Cell pellets and hemoglobin absorbance spectra of a K562 cell line expressing GFP in comparison to untransfected K562. Cells were incubated for 5 days at 37°C and 5 % CO₂ in K562 media. Grey curve: no media supplement. Red curve: supplement of 40 μM hemin. -: Untransfected K562. GFP: K562 expressing GFP. Cell pellets and spectra from one representative measurement are shown.