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Cell pellets and hemoglobin absorbance spectra of different K562 cell lines expressing the ER anchored HO-1 variant GFP-HO-1. Cells were incubated for 5 days at 37°C and 5 % CO2 in K562 media. Grey curve: no media supplement. Red curve: supplement of 40 μM hemin. -: Untransfected K562. 1-6: K562 expressing GFP-HO-1. Cell pellets and spectra from one representative measurement are shown.
