Table 1. Validation of MA expression data by qPCR.
| Gene (Symbol) | Pearson correlation MA / qPCR | p-value | Spearman correlation to survival | p-value |
|---|---|---|---|---|
| FCGR1B | 0.68 | < 0,0001 | -0.52 | 0.0023 |
| ANXA2 | 0.47 | 0.0059 | -0.46 | 0.0065 |
| PEBP1 | 0.46 | 0.0064 | 0.46 | 0.0074 |
| IMPA2 | 0.45 | 0.0086 | -0.34 | 0.0407 |
| MNDA | 0.58 | 0.0006 | −0.31 | 0.055 |
| SLC4A7 | 0.49 | 0.004 | 0.29 | 0.0679 |
| BST1 | 0.45 | 0.0078 | −0.26 | 0.0898 |
| MGST1 | 0.48 | 0.0048 | −0.24 | 0.1074 |
| ZNF467 | 0.38 | 0.0244 | 0.09 | 0.3288 |
| OAS2 | 0.42 | 0.0136 | −0.02 | 0.4637 |
| UBE2L6 | 0.33 | 0.0428 | 0.02 | 0.4681 |
| ATP1B3 | 0.20 | 0.1505 | NA | NA |
| FH | 0.16 | 0.2113 | NA | NA |
| LILRA5 (primer 1)a | 0.31 | 0.0527 | NA | NA |
| LILRA5 (primer 2)a | 0.28 | 0.0779 | NA | NA |
| TPM3 (probe 1)b | −0.16 | 0.2147 | NA | NA |
| TPM3 (probe 2)b | −0.10 | −0.0967 | NA | NA |
| SRSF6 | 0.01 | 0.4705 | NA | NA |
| MAT2B | −0.04 | 0.4255 | NA | NA |
| PSMB9 | −0.11 | 0.2933 | NA | NA |
| TLR6 | 0.14 | 0.2389 | NA | NA |
For the total 28 samples from stage IV patients of the Nijmegen discovery cohort we calculated the correlation between the expression levels as measured by microarray analysis (MA) against those obtained by qPCR. For validated genes we also calculated the correlation between qPCR expression and survival time. Only FCGR1B, ANXA2, PEBP1 and IMPA2 were validated (in bold). a Two different primers were tested. bTwo probes for this gene were put forward by our statistical pipeline. See also Supplementary Figures 2 and 3 for correlation plots. NA: Not analyzed.