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. 2017 Jun 27;8(40):67567–67591. doi: 10.18632/oncotarget.18747

Figure 2. Secreted CPE induces RPS6 phosphorylation.

Figure 2

(A-C) Signaling profiling of 7 GBM cell lines. Immunoblot detection of (A) sCPE in the supernatants and (B) 4EBP1 and (C) RPS6 levels and its phosphorylation in the lysates derived from 7 GBM cell lines. Ponceau staining and α-tubulin were used as loading controls for the supernatants and lysates, respectively. The cells were serum-starved for 24h in serum-reduced medium prior to supernatant collection and lysis. Representative immunoblots are shown. (D-F) Signaling profiling of LNT229 Neo-control and sCPE-overexpressing cells upon inhibition of the protein transport. Detection of (D) sCPE in the supernatants and (E) CPE or (F) RPS6 (total and phosphorylated forms) in the lysates cells. The cells were serum-starved for 5h in serum-reduced medium (without treatment) or for 5h and 10h in serum-reduced medium with 1x protein transport inhibitor (PTI) cocktail prior to supernatant collection and lysis. Representative immunoblots are shown.