Table 2. Hypophosphorylated proteins in LNT229 glioma cells upon sCPE overexpression.
| H/L | A.a. | Position | Loc.Prob. | Protein Name |
|---|---|---|---|---|
| −3.39 | S | 4 | 1.00 | Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) |
| −2.62 | S | 66;73;78;71;60;225;65 | 0.60 | LIM and calponin homology domains-containing protein 1 (LIMCH1) |
| −2.62 | S | 67;74;79;72;61;226;66 | 0.60 | LIM and calponin homology domains-containing protein 1 (LIMCH1) |
| −2.62 | S | 72;79;84;77;66;231;71 | 0.62 | LIM and calponin homology domains-containing protein 1 (LIMCH1) |
| −2.51 | S | 224;126 | 0.99 | Trans-Golgi network integral membrane protein 2 (TGOLN2) |
| −2.15 | S | 390;439;477;556;594 | 0.93 | Transcriptional regulator ATRX |
| −2.15 | S | 392;441;479;558;596 | 0.55 | Transcriptional regulator ATRX |
| −1.98 | S | 74;81;86;79;68;233;73 | 0.70 | LIM and calponin homology domains-containing protein 1 (LIMCH1) |
| −1.95 | S | 123 | 1.00 | RING1 and YY1-binding protein (RYBP) |
| −1.91 | S | 366 | 1.00 | Protein CASC4 |
| −1.91 | S | 374 | 1.00 | Protein CASC4 |
| −1.91 | S | 696;266 | 0.88 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A containing DEAD/H box 1 (SMARCAD1) |
| −1.91 | Y | 703;273 | 0.97 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A containing DEAD/H box 1 (SMARCAD1) |
| −1.51 | S | 378 | 1.00 | Integrator complex subunit 12 (INTS12) |
| −1.50 | S | 181;191 | 1.00 | Golgi membrane protein 1 (GOLM1) |
| −1.30 | S | 167;166;69 | 1.00 | ATP-binding cassette sub-family F member 1 (ABCF1) |
| −1.29 | S | 55 | 0.85 | Signal-induced proliferation-associated protein 1 (SIPA1) |
| −1.28 | S | 317 | 1.00 | Cyclin-dependent kinase 13 (CDK13) |
| −1.28 | S | 325 | 0.97 | Cyclin-dependent kinase 13 (CDK13) |
| −1.28 | S | 1785 | 0.99 | Microtubule-associated protein 1B (MAP1B) |
| −1.26 | S | 39;134 | 0.62 | LEM domain-containing protein 2 (LEMD2) |
| −1.26 | S | 44;139 | 0.85 | LEM domain-containing protein 2 (LEMD2) |
| −1.24 | S | 50;357;480;485 | 1.00 | Apoptosis-stimulating of p53 protein 2 (TP53BP2) |
| -1.23 | S | 512;589 | 1.00 | Kinesin light chain 2 (KLC2) |
| −1.22 | S | 1779 | 0.97 | Microtubule-associated protein 1B (MAP1B) |
| −1.20 | S | 180 | 0.57 | Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) |
| −1.19 | S | 152 | 0.84 | YTH domain-containing protein 1 (YTHDC1) |
| −1.18 | S | 58;65;70;63;52;217;57 | 1.00 | LIM and calponin homology domains-containing protein 1 (LIMCH1) |
| −1.18 | T | 63;68;61;50;215;55;56 | 0.99 | LIM and calponin homology domains-containing protein 1 (LIMCH1) |
| −1.18 | S | 24;148;449;456;486;488;508;525;542 | 0.76 | Histone deacetylase 7 (HDAC7) |
| −1.16 | S | 102 | 0.73 | PC4 and SFRS1-interacting protein (PSIP1) |
| −1.16 | S | 105 | 0.67 | PC4 and SFRS1-interacting protein (PSIP1) |
| −1.16 | S | 196;202;207 | 1.00 | Caldesmon (CALD1) |
| −1.16 | S | 977 | 1.00 | Protein-methionine sulfoxide oxidase MICAL3 |
| −1.15 | S | 1782 | 0.99 | Microtubule-associated protein 1B (MAP1B) |
| −1.13 | S | 1503;1529;1405;1431;238;265 | 0.54 | Centrosomal protein of 170 kDa (Cep170) |
| −1.10 | S | 24;109 | 0.86 | Transcriptional coactivator YAP1 |
| −1.10 | S | 11;12;23;30 | 1.00 | Septin-9 (Sept9) |
| −1.10 | S | 1688 | 1.00 | Telomere-associated protein RIF1 |
| −1.08 | S | 283 | 1.00 | A-kinase anchor protein 8-like (AKAP8L) |
| −1.08 | S | 955;978;1009 | 0.51 | Tight junction protein ZO-2 (TJP2) |
| −1.08 | S | 956;979;1010 | 0.51 | Tight junction protein ZO-2 (TJP2) |
| −1.08 | S | 963;986;1017 | 0.98 | Tight junction protein ZO-2 (TJP2) |
| −1.06 | S | 126;171 | 1.00 | Polymerase I and transcript release factor (PTRF) |
| −1.05 | S | 562;568 | 0.75 | Serine/threonine-protein kinase N1 (PKN1) |
The table provides a list of hypophosphorylated proteins in CPE-overexpressing LNT229 versus corresponding Neo-control cells detected by SILAC followed by phosphoproteomic analysis. The phospho-proteins discussed in the manuscript are highlighted in bold. Abbreviations: H/L - log2 ratio of phospho-enrichment in “heavy”-labeled CPE-overexpressing LNT229 cells to “light”-labeled Neo-control LNT229 cells; A.a. - amno acid; Position - position of amino acid with detected phosphorylation; Loc.Prob. - localization probability.