Table 2. Performance of Proton and Digital PCR for detecting of EGFR mutation from tumor tissue DNA compared with ARMS PCR.
| EGFR mutation | ARMS | Proton | Performance of Proton | Digital PCR | Performance of Digital PCR | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Positive | Negative | Sensitivity (%) | Specificity (%) | Concordance (%) | Positive | Negative | Sensitivity (%) | Specificity (%) | Concordance (%) | ||
| L858R | Positive | 9 | 0 | 100% | 100% | 100% | 9 | 0 | 100% | 96.97% | 97.62% |
| Negative | 0 | 33 | 1 | 32 | |||||||
| 19 DEL | Positive | 14 | 0 | 100% | 89.29% | 92.86% | 13 | 1 | 92.86% | 89.29% | 90.48% |
| Negative | 3 | 25 | 3 | 25 | |||||||
| T790M | Positive | 2 | 0 | 100% | 97.50% | 97.62% | 2 | 0 | 100% | 60.00% | 61.90% |
| Negative | 1 | 39 | 16 | 24 | |||||||
| Total* | Positive | 25 | 0 | 100% | 94.17% | 96.83% | 24 | 1 | 96.00% | 80.20% | 83.33% |
| Negative | 4 | 97 | 20 | 81 | |||||||
*. Comparison of Proton/Digital PCR with ARMS PCR was based on 42 tumor biopsies all tested in three platforms. Each EGFR mutation type had 42 sites’ result, the sensitivity and specificity were counted take site as unit.