Figure 3. Generation of specific ASM-expressing viruses.

(A) Schematic of generation of adeno-associated viruses (AAVs) carrying miR-384 (AAV-miR-384) or null (AAV-CTL) under control of an α-SMA promoter. (B) Visualization of the transduced ASM cells in culture by GFP. (C-D) The transduced cultured ASM cells were examined for miR-384 by RT-qPCR (C), and Beclin-1 by Western blotting (D). *p<0.05. N=5. Scale bars are 20 μm.