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. 2017 Aug 4;8(40):68208–68220. doi: 10.18632/oncotarget.19919

Figure 4. Sorafenib inhibits BV6/TNFα-induced assembly of the necrosome in acute leukemia cells.

Figure 4

(A) FADD-deficient Jurkat cells were treated for 2 hours with 1 μM BV6 and 1 ng/ml TNFα in the presence or absence of 10 μM Sorafenib, 6 μM GSK’872 or 0.6 μM Dabrafenib. RIP1 was immunoprecipitated using anti-RIP1 antibody and detection of indicated proteins was carried out by Western blotting; *: IgG heavy chain. (B) FADD-deficient Jurkat cells were treated for 2 hours with 1 μM BV6 and 1 ng/ml TNFα in the presence or absence of 10 μM Sorafenib, 10 μM GSK’872 or 5 μM Dabrafenib. RIP1 was immunoprecipitated using anti-RIP1 antibody and detection of indicated proteins was carried out by Western blotting; *: IgG heavy chain. (C) FADD-deficient Jurkat cells were treated for 2 hours with 1 μM BV6 and 1 ng/ml TNFα in the presence or absence of 10 μM Sorafenib, 6 μM GSK’872 or 0.6 μM Dabrafenib. RIP1 was immunoprecipitated using anti-RIP1 antibody crosslinked to beads and detection of indicated proteins was carried out by Western blotting.