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. 2017 Aug 4;8(40):68221–68229. doi: 10.18632/oncotarget.19920

Figure 1. PD-L1 and MET gene expression and PD-L1 cell surface expression in HCC827ER.

Figure 1

(A) MET gene expression was measured at each concentration-point during establishment of the resistant cell line (the erlotinib concentrations are indicated at the x-axis), and normalized to the expression in the HCC827PAR cell line. Initially expression is decreased, but during resistance development expression starts increasing (approximately from 200 nM erlotinib), and markedly exceeds that level of the HCC827PAR when resistance is established. (B) Correspondingly, PD-L1 gene expression was measured during the resistance-development, and normalized to HCC827PAR. PD-L1 expression also starts increasing at approximately 200 nM erlotinib, and expression in the final erlotinib-resistant cell line exceeds that of HCC827PAR. (C) Using flow cytometry the PD-L1 protein expression was measured. A representative histogram and mean fluorescence intensity (MFI) values are presented.