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. 2017 Jul 17;8(40):68252–68262. doi: 10.18632/oncotarget.19291

Figure 4. The miR-138-PKM2pathwayregulates TSCC cell migration/invasion.

Figure 4

(A) Predicted target sequences for miR-138 are located in the 3’-UTR of PKM2 mRNA. (B) Dual luciferase reporter assays were performed to evaluate the target genes of miR-138. Following transfection with pGL-PKM2 for 24 h, luciferase activity was significantly reduced in cells treated with miR-138 mimics relative to cells treated with control mimics (*P<0.05 vs blank). After the seed region of the target site was mutated (pGL-PKM2 m), the effects of miR-138 on luciferase activity were abolished. (C, D) Western blot results showing the levels of the PKM2, SOD2, pERK, ERK and EMT marker (E-cad, Vimentin,and Slug) proteins in TSCC cells transfected with the miR-138 mimic (C) or miR-138 LNA (D).