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. 2017 Jul 17;8(40):68270–68279. doi: 10.18632/oncotarget.19295

Figure 3. Increased migratory and invasive potential of LL/2-R cell subline.

Figure 3

(A) A wound healing assay showed that LL/2-R cells had increased migratory ability. Representative images are shown imediately after a scratch was created (0h) and 48h later. Scale bars, 100μm (×100). (B) LL/2-R cells had increased ability to invade through Matrigel-coated transwell membranes. After 48 hours, the invaded cells were stained, photographed and counted. Representative photographs of transwell membranes showed stained invaded cells. Columns, mean of three independent experiments; bars, SD; *, P<0.05; scale bars, 50μm(×100). (C) Western blotting assay was used to investigate MMP-2 and MMP-9 levels of LL/2 cells and tumors, with GAPDH as a loading control. The MMP2 and MMP9 levels of LL/2-R cells and tumors were upregulated, compared with LL/2-P. The expression of MMP2 and MMP9 in LL/2-R cells seems increased when treated with climbing concentration of sunitinib for 48h from 5μM to 20μM. Three independent experiments were conducted.