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. 2017 Jul 31;8(40):68517–68529. doi: 10.18632/oncotarget.19704

Figure 2. SAMHD1 is acetylated at K405 by ARD1.

Figure 2

(A) Construction of SAMHD1 deletion mutants (left). SAM; SAM domain, HD; HD domain, CTD; C-terminal domain. Deletion mutants of GST-SAMHD1 were subjected to in vitro acetylation assays with ±His-ARD1 and blotted with anti-Lys-Ac antibody (right). Arrows indicate respective proteins. (B) GST-CTD were acetylated in vitro and subjected to LC–MS/MS. (C) GST-SAMHD1 recombinants were subjected to in vitro acetylation assays with His-ARD1. Acetylation levels were determined with an anti-Lys-Ac antibody. (D) FLAG-SAMHD1 variants were immnunoprecipitated from HEK293T cells, and their acetylation levels in vivo were analyzed using an anti-Lys-Ac antibody. (E) Sequence alignment of SAMHD1 K405 residue in various species.