Skip to main content
. 2017 Jul 31;8(40):68517–68529. doi: 10.18632/oncotarget.19704

Figure 4. SAMHD1 acetylation is important for cell proliferation in various cancer cells.

Figure 4

(A) Tumor and non-tumor tissues from 7 hepatocarcinoma patients were lysed and extracted. Protein expression levels were analyzed by western blot and presented as mean ±S.E.M. (n=7). T, tumor tissue; N, non-tumor tissue. (B) Cell growth of siCtrl or siSAMHD1 treated HeLa cells were monitored over time by MTS assay. Shown are mean ±S.E.M. (n=4). (C) siCtrl or siSAMHD1 treated MCF7 cells were subjected to anchorage-dependent colony formation assays. Number of colonies were presented as mean ±S.D. (n=3) with representative well pictures. (D) Cell growth of stable HeLa cells expressing SAMHD1 wildtype, K405R or empty vector were monitored over time by MTS assay. Shown are mean ±S.E.M. (n=4). (E) Stable MCF7 cells expressing SAMHD1 wildtype, K405R or empty vector were subjected to anchorage-dependent colony formation assays. Number of colonies were presented as mean ±S.D. (n=3) with representative well pictures.*P<0.05; **P<0.01; ***P<0.001; t test; N.S.; not significant.