Figure 8. TGF-β1 increases defense against bacteria by CYP27B1-dependent activation.

(A) 16HBE cells were pretreated with TGF-β1 and TGF-β1/25VD₃. (B) 16HBE cells were pretreated with ITRA. The cells infected with P. aeruginosa cells were lysed and plated to confirm the presence of intracellular bacteria. Results are representative of three experiments and data are shown as means±SDEV. One-way ANOVA for group comparisons. Significant differences in the mean values were defined as p < 0.05. (C) Schematic representation of TGF-β1 induced CYP27B1 role in HDM immunotherapy increasing defense against P. aeruginosa during allergic airway inflammation.