FIGURE 2:

Protection by astrocytes is not due to removal of AβOs from media. (A) Dot blots of astrocyte lysates showed readily detectable oligomer immunoreactivity when obtained from cultures incubated with 500 nM AβOs. (B) Uptake is time dependent and plateaus at ∼5 min. (C) AβO levels in media (500 nM) were unchanged by the presence of astrocyte feeder layers over a 30-min period (red bar; astrocyte-free control conditions, black bar). MEM culture media contained 500 nM AβOs. *, p < 0.05, Mann-Whitney; n.s., p = 0.30, unpaired t test. (D) Examples of dot immunoblot signals quantified in A–C.