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. 2017 Sep 13;49(3):176–183. doi: 10.3947/ic.2017.49.3.176

Table 1. Primers and amplification conditions for PCR assay.

Target gene Primer pair sequences
5'→3'
PCR product (bp) PCR condition Reference
ddlE.faecalis ATCAAGTACAGTTAGTCT 941 94℃ 60 s, 54℃ 60 s [9]
ACGATTCAAAGCTAACTG 72℃ 60 s, 30 cycles
vanA CATGAATAGAATAAAAGTTGCAATA 1,030 94℃ 60 s, 54℃ 60 s [15]
CCCCTTTAACGCTAATACGATCAA 72℃ for 60 s, 30 cycle
vanB GTGACAAACCGGAGGCGAGGA 433
CCGCCATCCTCCTGCAAAAAA
esp AGATTTCATCTTTGATTCTTGG 510 94℃ 60 s, 56℃ 60 s [16]
AATTGATTCTTTAGCATCTGG 72℃ 60 s, 30 cycles
cyl ACTCGGGGATTGATAGGC 688
GCTGCTAAAGCTGCGCTT
gelE TATGACAATGCTTTTTGGGAT 213
AGATGCACCCGAAATAATATA

PCR, polymerase chain reaction; bp, base pair