Table 1. Primers and amplification conditions for PCR assay.
Target gene | Primer pair sequences 5'→3' |
PCR product (bp) | PCR condition | Reference |
---|---|---|---|---|
ddlE.faecalis | ATCAAGTACAGTTAGTCT | 941 | 94℃ 60 s, 54℃ 60 s | [9] |
ACGATTCAAAGCTAACTG | 72℃ 60 s, 30 cycles | |||
vanA | CATGAATAGAATAAAAGTTGCAATA | 1,030 | 94℃ 60 s, 54℃ 60 s | [15] |
CCCCTTTAACGCTAATACGATCAA | 72℃ for 60 s, 30 cycle | |||
vanB | GTGACAAACCGGAGGCGAGGA | 433 | ||
CCGCCATCCTCCTGCAAAAAA | ||||
esp | AGATTTCATCTTTGATTCTTGG | 510 | 94℃ 60 s, 56℃ 60 s | [16] |
AATTGATTCTTTAGCATCTGG | 72℃ 60 s, 30 cycles | |||
cyl | ACTCGGGGATTGATAGGC | 688 | ||
GCTGCTAAAGCTGCGCTT | ||||
gelE | TATGACAATGCTTTTTGGGAT | 213 | ||
AGATGCACCCGAAATAATATA |
PCR, polymerase chain reaction; bp, base pair