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. 2017 Sep 26;9(9):159–168. doi: 10.4252/wjsc.v9.i9.159

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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.

Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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Enrichment of cancer stem cells using the sphere culture method. A: Photomicrographs of Hepa 1-6 3-dimensional spheres on day 5 cultured in stem cell conditioned serum-free medium; B: Hepa 1-6 sphere sizes and numbers were enumerated; C: Photomicrographs of AML12 3-dimensional spheres on day 5; D: AML12 spheres sizes and numbers were enumerated.