Figure 4.

The central conserved region (CCR) of RSV G protein alters IFNλ expression. Polarized LCC Calu-3 cells were infected with RSV/A2, rA2-GC12 or rA2-GC4 virus (MOI = 1.0). Total RNA was isolated from mock and infected cells at 12 h and 24 h pi. Expression of IFNα1, IFN λ, SOCS-1 and SOC-3 were determined by gene specific primer-probe combinations using a one-step qRT-PCR assay at 12 h (a) or 24 h (b) pi Data represent ± SEM of three independent experiments. ** indicates statistical significance (p < 0.01) and **** indicates statistical significance (p < 0.001) as determined by two-way ANOVA and the Bonferroni post hoc test.