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. 2017 Sep 12;5(3):57. doi: 10.3390/microorganisms5030057

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Hyaluronic acid fragments produced by the activity of HYLs of P. acnes. HA was incubated with controls and culture supernatants of P. acnes strains grown to the stationary phase. Visualization of HA fragments was done on a stained 1% agarose gel. HYL-IA produced larger HA fragments, whereas HYL-IB/II activity resulted in small HA fragments. Lanes: 1, HA input; 2, positive control (HYL from S. pyogenes); 3, culture medium (control); 4; strain 266 (IA); 5, strain 12.1.L1 (IA); 6, strain 15.1.R1 (IA); 7, strain KPA171202 (IB); 8, strain CCUG33950 (II); 9, strain PMH5 (III); 10, strain ∆hyl mutant. The picture is representative of three independent experiments.