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. Author manuscript; available in PMC: 2018 Apr 15.
Published in final edited form as: Cancer Res. 2017 Feb 15;77(8):2124–2133. doi: 10.1158/0008-5472.CAN-15-2870

Figure 2. Surviving crypt and Lgr5+ CESC kinetic profile after 19 Gy distal colon irradiation.

Figure 2

(A) Technique of distal colon irradiation. Schematic presentation of a mouse positioned for irradiation, and the plots of dose uniformity anteroposterior and lateral across the irradiated volume as described in Methods. (B, C) Mice were treated with 19 Gy targeted to the distal colon. In (B), fur loss at 40 days delineates the site of the incident beam at the skin level with sharp margin. In (C), radiation damage to the distal colon mucosa is confirmed by histology at day 5 after 19 Gy (described in Figure 1B) as restricted to the dotted region within the standardized Swiss Roll. (D) LacZ staining of distal colons of Lgr5-lacZ mice treated with 19 Gy distal colon irradiation at day 0, 5, 7 and 10. All images are of the same magnification; scale bar = 50 µm. (E) Quantification of surviving crypts (upper) and Lgr5+ stem cells (lower) in distal colon sections of Lgr5-lacZ mice irradiated with 19 Gy at day 0, 5, 7 and 10. Data (mean ± standard deviation) were quantified from 4 mice/time point using 3–5 circumferences/mouse.