Fig. 4. Effect of ASO binding sites on first- and second-step RNA splicing efficiency. (a) Diagrammatic representation of ASO targeting sequences (5′-ASO, Mid1-ASO, Mid2-ASO, BP-ASO and 3′-ASO). Numbering starts from the first position of intron 14. The 5′ss and 3′ss are indicated by vertical arrows. The branchpoint is indicated in red. (b) Fluorescence intensity of free 5′-exon and spliced mRNA produced in the splicing reaction by adding different ASOs. (c) Schematic diagram of the splicing block caused by ASOs binding different sites.
