Fig. 5. Analysis of 5′-ASO and 3′-ASO regulated RNA splicing processes. (a and b) Gel electrophoresis of the RT-PCR assay: in vitro RNA splicing was performed in NE in the presence of increasing amounts of 5′-ASO or 3′-ASO (0 pM, 1 pM, 5 pM, 10 pM, 20 pM and 40 pM). (c and d) Fluorescence responses in different concentrations of 5′-ASO or 3′-ASO (0 pM, 1 pM, 5 pM, 10 pM, 20 pM and 40 pM).
