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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Bone. 2017 Mar 24;100:100–109. doi: 10.1016/j.bone.2017.03.047

Fig. 8.

Fig. 8

αKlotho increases endogenous antioxidants in A549 lung epithelial cells. A. Hydrogen peroxide (H2O2) activates and αKlotho further activates the ARE reporter in a dose-dependent manner measured by firefly/renilla luciferase assay. P < 0.05: * vs. 0 αKlotho. Ɨ vs. (H2O2 + 0 Klotho). B. Addition of αKlotho ameliorates DNA (8-OHdG), protein (carbonyl) and lipid (8-isoprostane) oxidative damage caused by H2O2. All data points are statistically different from baseline (H2O2 + 0 αKlotho). C. Immunoblots of selected candidates of the endogenous Nrf2 antioxidant network. MTA: methalothionine, HOX1 or 2: Heme oxygenase 1 or 2, PXDN: peroxidasin. Based on data from [55].