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. 2017 Oct;23(10):1737–1739. doi: 10.3201/eid2310.161855

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This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Growth curve and phylogenetic analysis of RJ-IB1 and RJ-IB5 CHIKV isolates, Rio Janeiro, Brazil, 2016. A) Vero cell–amplified RJ-IB1 or RJ-IB5 was titrated and used to infect Vero cells in duplicates at a multiplicity of infection of 0.05. The resulting supernatants were harvested at 0, 24, 48, and 72 h after infection. The production of infectious progeny was determined by plaque assay in Vero cells. B) The full-genome sequences of RJ-IB1 and RJ-IB5 isolates and 53 CHIKV strains representing all known genotypes were aligned with MAFFT (http://mafft.cbrc.jp/alignment/software). Phylogeny inference was performed with MEGA 6 (http://www.megasoftware.net) opting for the neighbor-joining method and kimura-2p model of substitution. Numbers on branches indicate the percentage of bootstrap support from 1,000 replicates. Values >70% are shown. Similar tree topology was obtained with maximum-likelihood method opting for Tamura-Nei model of substitution. Isolates are identified by GenBank accession number, location, and year of CHIKV isolation; genotypes are indicated at right. CHIKV, chikungunya virus; ESCA, East/Central/South African genotype. Scale bar indicates nucleotide substitutions per site.