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. Author manuscript; available in PMC: 2018 Sep 27.
Published in final edited form as: Neuron. 2017 Sep 27;96(1):115–129.e5. doi: 10.1016/j.neuron.2017.09.003

Figure 7. ApoE4 Aggregation in Aging Brain Accelerates the Insolubility of IR.

Figure 7

RIPA and guanidine (GND)-extracted brain cortical lysates of apoE3-TR and apoE4-TR mice (n=4–6 mice/genotype, mixed gender) at 3 and 22 months of age were prepared and the amount of apoE and IR was examined by Western blotting (A–C) and ELISA (D–I). Results were normalized to β-actin levels in Western blot analysis. The ratios of insoluble (GND fraction) to soluble (RIPA fraction) apoE (F) and IR (I) were calculated. Data are expressed as mean ± SEM. Two-way ANOVA with Tukey’s multiple comparisons test was used for statistical analysis. **p < 0.01; ***p < 0.001, N.S., not significant. Molecular mass markers in kilodaltons are shown.