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. 2017 Sep 29;12(9):e0185516. doi: 10.1371/journal.pone.0185516

Fig 7. Cellular concentration and phosphorylation pattern of Rgt1 under repressing and activating growth conditions.

Fig 7

The WT (MB159-4D) and rpc128-1007 strain expressing HA-tagged Rgt1 were grown to A600 ≈1.0 in 2% glucose or 2% glycerol-rich medium. Total cell protein extracts were subjected to SDS-PAGE and hybridized to anti-HA antibodies (A). The quantified relative level of Rgt1-HA (B) protein compared to Vma2 protein was quantified for three independent experiments in triplicate with standard deviations. The molecular weight (MW) of Rgt1-HA was ~144.7 kDa, and the MW of Vma2 was ~57.7 kDa.