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. 2017 Sep 29;12(9):e0185516. doi: 10.1371/journal.pone.0185516

Fig 8. A low cellular concentration of Tup1 in the rpc128-1007 mutant with low RNAP III activity.

Fig 8

The WT (MB159-4D) and rpc128-1007 strains expressing HA-tagged Tup1 proteins were grown to A600 ≈ 1.0 on 2% glucose or 2% glycerol-rich medium. Total cell protein extracts were subjected to SDS-PAGE and hybridized to anti-HA antibodies (A). The relative level of Tup1 (B) protein in comparison to Vma2 protein was quantified for three independent experiments conducted in triplicates with standard deviations. The molecular weight (MW) of Tup1 was ~89.88 kDa, and the Vma2 MW was ~57.7 kDa.