Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 14;13(9):e1006991. doi: 10.1371/journal.pgen.1006991

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

PMC Copyright notice

Fig 2 — (A) Hematoxylin and eosin-stained femurs sections from wild-type and Thra1^PV/+ mice (a and b). Magnification = X330. (c). The numbers of total bone marrow cells were counted from wild-type (panel a; n = 20) and Thra1^PV/+ mice (panel b; n = 18). (d). The fat areas in the bone marrow of wild-type mice (n = 3) and Thra1^PV/+ mice (n = 3) shown in Figure A-a and A-b were measured by morphometry. Values are means ± SEM (n = 3). (B) The distribution of respective erythroid and megakaryocytic populations in the bone marrow of wild type and Thra1^PV/+ mice. Erythroid and megakaryocytic progenitors and mature cells were analyzed by FACS using cell surface markers. CFU-E erythrocyte progenitors identified in the bone marrow cells that had characteristics of Lin-Ter119-cKit+CD71+ (the pink box in panel a). Erythrocytes identified as CD71-Ter119+ bone marrow cells (the red box in panel b). Colony-forming units-megakaryocytes (CFU-Mk) megakaryocytic progenitors identified as Ter119-Sca1-CD41+CD61+cKit+ bone marrow cells (the orange box in panel c). Megakaryocytes were identified as Ter119-Sca1-CD41+CD61+cKit- bone marrow cells (the blue box in panel c). The numbers of CFU-E cells (panel d), erythrocytes (panel e), CFU-Mk cells (panel f), and megakaryocytes (panel g) were counted and the data calculated as fold changes in Thra1^PV/+ versus wild-type mice. The representative data from three experiments are shown. Values are means ± SEM (n = 3). The p values are shown. (C) In vitro colony forming assays of bone marrow cells from wild-type and Thra1^PV/+ mice (panels a-c). Morphological characteristics of burst-forming units-erythroid (BFU-E) progenitors (panel a), CFU-E progenitors (panel b), and CFU-Mk progenitor (panel c) colonies by phase contrast microscopy. Panels d, e, and f show the scored colonies of the BFU-E, CFU-E, and CFU-Mk. BFU-E was counted after 14 days. CFU-E was counted after 2 days. CFU-Mk was counted after 7 days in vitro culturing. The data are presented as ratios of total number of colonies versus total bone marrow cells. Values are means ± SEM (duplicates in each assay; WT mice, n = 7; Thra1^PV/+ mice, n = 6). * denotes p<0.05; ** denotes p<0.01, *** p<0.001. (D). Morphological characteristics of multi-potential (CFU-GEMM) progenitor colonies (panel a), granulocyte/macrophage progenitor cells (CFU-GM) (panel c) by phase contrast microscopy (x100). Panels b and d show the number of colonies of CFU-GEMM and CFU-GM, respectively. The colonies were counted after 8 days in vitro culturing as described in Methods. The data are presented as number of colonies versus total bone marrow cells. Values are means ± SEM (WT mice, n = 4; Thra1^PV/+ mice, n = 4; quadruplicate in each assay).