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. 2017 Sep 14;13(9):e1006991. doi: 10.1371/journal.pgen.1006991

Fig 7. Thyroid hormone response elements in the Gata1 promoter mediates the transcription of TRα1.

Fig 7

Schematic representation of the Gata1 promoter with TRE1 downstream of TSS site and TRE2 together with GATA box and two E-boxes upstream of the TSS (A-I-a), the luciferase reporter construct containing TRE1 (A-I-b), the luciferase reporter construct containing TRE2 (A-I-c). Reporter activity mediated by a Palindromic TRE (Pal-Luc) (A-II-a), TRE1 (A-II-b) and TRE2 (A-II-c) in K562 cells expressing endogenous TR (bars 1–2) and with transfected TRα1PV(bars 3–4), with cells transfected with TRα1 without TRα1PV (bars 5–6) or with TRα1PV (bars 7–8), in the absence or presence of T3. Values are means ± SEM (n = 3). (B) Effects of thyroid hormone on the expression of the Gata1 (panel a, n = 4) and the Klf1 gene (panel b; n = 3). The treatment of mice with PTU with or without T3, to make mice hypothyroid or hyperthyroid, was the same as described in Fig 3. Values are means ± SEM (n = 3). * denotes p<0.05; ** denotes p<0.01; *** p<0.001.