Fig 8. Defective terminal erythropoiesis was rescued by overexpression of the GATA1 gene.

(A) Expression of GATA1 tagged with V5 was determined in the bone marrow cells by western blot analysis using anti-V5 antibodies. Anti-GAPDH antibody served as the loading control. (B). Terminal erythropoiesis was determined by flow cytometry using a late erythroid surface phenotype (Ter119+FSC^low, boxed in red) after transfection of control (panel a) or GATA1 expression plasmids (panel b) in wild-type (B) and Thra1^PV/+ mice (C). The Fold changes of matured erythrocytes after transfection of GATA1 gene in WT mice (B, panel c) and Thra1^PV/+ mice (C, panel c) are indicated. Values are means ± SEM (n = 3). * denotes p<0.05.