Fig 9. Expression of erythroid genes was increased by overexpression of the GATA1 gene in the bone marrow of Thra1PV/+ mice.
After transfection of V5-tagged GATA1 plasmids into bone marrow cells, mRNA expression of erythroid genes was analyzed by quantitative real-time PCR (panel A-G). mRNA expression of Gata1 (panel A), Klf1 (panel B), β-globin (panel C), dematin (panel D), CAII (panel E), band 3 (panel F) and eALAS (panel G) was increased in the bone marrow of Thra1PV/+ mice (lanes 4) compared with the empty vector control (lanes 3). Values are means ± SEM (n = 3). NS: not significant; * denotes p<0.05; ** denotes p<0.01; *** p<0.001.