Skip to main content
. Author manuscript; available in PMC: 2017 Sep 29.
Published in final edited form as: J Immunol. 2015 Aug 28;195(7):3171–3179. doi: 10.4049/jimmunol.1501132

Figure 6. Rescue of sensitivity to ionizing radiation.

Figure 6

Fibroblasts from compound heterozygous SCID (n=2) and normal (n=3) littermates were transfected with human Artemis-expressing plasmid (5 μg, Artemis), with a molar equivalent of pExodus plasmid without the Artemis gene (3.45 μg, Empty Vector), or shocked without plasmid added. Fibroblasts were exposed to a 4 Gy radiation dose 24 hours after transfection. Colonies (≥ 2 mm) were counted after 14 days of growth. The average number of surviving colonies for three replicates for a given plasmid was divided by the number of colonies from shock only cells for each animal. Error bars represent the standard error of the least squares means. Dots show individual observations. Bars with different letters within affected status (a and b) represent statistical differences between means with p<0.01.