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. Author manuscript; available in PMC: 2017 Sep 29.
Published in final edited form as: Cell Rep. 2017 Sep 26;20(13):3176–3187. doi: 10.1016/j.celrep.2017.09.006

Figure 6. Lymphatic endothelial cells are activated following infection and allow for dendritic cell migration to draining lymph nodes.

Figure 6

Wildtype C57BL/6 mice were infected by cutaneous scarification with VACV-GP33. (A) Representative flow cytometry (gated on CD45−CD31+) of cutaneous endothelial cells at 0, 3 and 7 days post infection. (B) Quantification of activation markers on blood endothelial cells (BEC; CD45−CD31+gp38−) and lymphatic endothelial cells (LEC;CD45−CD31+gp38+) (n=6). (C) Quantification of CCL21 in draining lymph nodes (dLN) (n=4). (D) Quantification of migratory DCs present in dLNs following cutaneous VACV infection at the indicated time points or 24 hours following administration of dibutyl phthalate (DBP). (E) Quantification of FITC+ migratory DCs present in dLNs. 5% FITC dissolved in DMSO:Acetone painted on skin 24 hours prior to each time point. FITC dissolved in the skin irritant dibutyl phthalate (DBP):acetone used as a positive control. Statistics determined by students unpaired t test. *p<0.05, **p<0.01, ***p<0.001. Each point is an individual mouse.