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. 2017 Aug 17;2(16):e90103. doi: 10.1172/jci.insight.90103

Figure 7. Effects of doxycycline and rapamycin on Prox1 and VEGFR-3 in lymphatics of normal mice.

Figure 7

(A and B) Confocal micrographs comparing Prox1 (A) and VEGFR-3 (B) staining of lymphatics in the trachea of control mouse (left, single-transgenic CCSP) and a CCSP/VEGF-C mouse (right) after doxycycline (7 days, P21–28). Images were photographed with matching brightness and contrast settings. (C–E) Intensity of Prox1 fluorescence (C), number of Prox1-positive nuclei (D), and intensity of VEGFR-3 fluorescence (E) under same conditions as in A and B. *P < 0.05 vs. single-transgenic control, ANOVA; n = 5–6 mice/group. (F–I) Confocal micrographs comparing Prox1 (F) and VEGFR-3 (G) fluorescence in lymphatics and corresponding measurements (H and I) in normal mice after treatment with vehicle or rapamycin (2 days, P21–P23). Scale bar: 100 μm. Box and whisker plots show the median, first and third quartiles, and maximum and minimum.