Figure 4. Somatic mutations in IGHV3-23 and the effect of positions involved in binding on the affinities of hmAb 1E01.

(A) Frequency of amino acid substitution along the IGHV3-23 gene segment of DGP-reactive and non–DGP-reactive PCs from a representative CD patient with more than 30 sequences available for analysis. (B) Binding of DGP by single amino acid substituted variants S64-H (B, left panel) and Y66-H (B, right panel) of hmAb 1E01 as assessed by MST (average of 3 measurements ± SD).