Figure 1. Exome-capture RNA sequencing of aged, FFPE, and decalcified tumors.

(A) ecRNA-seq quality metrics (GC content, insert size, gene body coverage, and cumulative gene assignment diversity) of aged and tumor-matched, formalin-fixed, paraffin-embedded (FFPE) and flash-frozen (FF) samples. FF samples in blue, FFPE samples in red (n = 4 pairs). (B) Expression value correlations between four sets of matched tumor samples (FF vs. FFPE), along with Pearson r correlations and sample ages. (C) ecRNA-seq quality metrics of matched nondecalcified and decalcified samples. Nondecalcified samples in blue, decalcified samples in red (n = 3 pairs). (D) Expression correlations between three sets of matched tumor samples (nondecalified vs. decalcified), along with Pearson r correlations.