Skip to main content
. 2017 Sep 7;2(17):e91068. doi: 10.1172/jci.insight.91068

Figure 7. RNA-Seq reveals widespread reversal of hypertrophic transcription by muscle enhancer factor-2 (MEF2) silencing.

Figure 7

Analysis pipeline is described in Methods. (A) Unsupervised hierarchical cluster analysis of the cardiac transcriptome after transverse aortic coarctation (TAC) or a sham operation followed by 4 weeks of daily treatment with 8MI or its vehicle (blank) by gavage. Differentially up- and downregulated genes in left ventricle are shown; 1 column = 1 mouse; n = 3 per group. Visualization and analysis were done in GENE-E (https://software.broadinstitute.org/GENE-E/index.html). The full data set is provided as Supplemental File 2. (B) Expanded view of 4 selected clusters. See gene annotations in Supplemental Table 3. (C) Ingenuity Network analysis implicates MEF2 as 8MI target. See Supplemental Table 4 for complete set of identified networks. (D) 8MI transforms the transcriptional response to stress. Note little overlap of stress-regulated transcripts between 8MI and blank-treated mice. Venn diagrams generated using tools at VIB, Ghent University (http://bioinformatics.psb.ugent.be/webtools/Venn/).