Figure 3. Tead1 is required for maintaining cardiomyocyte SERCA2a activity.

(A) Serca2 protein level in control and Tead1-icKO hearts. (B) mRNA expression of genes related to Ca²⁺ cycling (n = 4). (C) ChIP from 12-week-old mouse hearts (n = 3) was performed with control mouse IgG (mIgG) and TEAD1 antibody. The presence of mSerca2a promoter was detected by qPCR. (D) mSerca2a promoter luciferase assay in H9c2 rat cardiomyoblasts. (E) SR-associated Ca²⁺-ATPase activity. (F) Representative Ca²⁺ tracing following 10 mmol/l caffeine stimulus of SR Ca²⁺ release. (G) Quantification of SR Ca²⁺ content (F1/F0). (n = 3, cell number = 15–17). ***P < 0.001, **P < 0.01, *P < 0.05; P values were determined using Student’s t test.