Figure 7. Mtg16^–/– tumors display altered intratumoral immune environment and increased DNA damage.

Skewing towards M2 macrophage phenotypes inMtg16^–/–tumors. (A) Representative images of IL-1β⁺/F4/80⁺ cells in WT and Mtg16^–/– tumors; white arrow heads indicate nonspecific staining, white arrows indicate dual staining. (B) Quantification of M1 macrophages per tumor high-power field (HPF) (WT = 12, Mtg16^–/– = 12). (C) Representative images of ARG1⁺/F4/80⁺ cells in WT and Mtg16^–/– tumors and (D) quantification of M2 macrophages per tumor HPF (WT = 12, Mtg16^–/– = 12). (E) DNA damage was assessed using 8-hydroxy-2-deoxyguanosine (8-OHdG) immunohistochemical staining. Representative images from tumors and (F) quantification of 8-OHdG⁺ cells in tumors (WT = 12, Mtg16^–/– = 13) and (G) crypts (WT = 12, Mtg16^–/– = 12, all representative images at ×40 magnification). Student’s t test, ***P < 0.001.