Figure 8. Wnt11 loss in hypoxemic TOF infants is associated with Rb1 suppression and induction of Plk1.

(A) Expression of Wnt11, Rb1, and Plk1 in TOF patients (qRT-PCR), n = 5 TOF cases were hypoxemic (O₂ saturation < 85%), and n = 3 cases with normal O₂ saturation (>95%). (B) Representative confocal images of anti–phospho-histone H3 (anti–p-H3) immunohistochemistry (IHC) in TOF infants. Arrows indicate representative p-H3–positive cardiomyocytes. Original magnification, ×60. Note: The studies presented in this figure were performed in a new group of TOF patients that were not included in the studies presented in Figure 4. Error bars represent SEM. *P ≤ 0.05 by unpaired, 2-tailed Student’s t test.