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. 2017 Sep 21;2(18):e93265. doi: 10.1172/jci.insight.93265

Figure 3. Additive effect of Aire deficiency and anti–CTLA-4 antibody on T cell responses.

Figure 3

(A and B) Tumor-infiltrating lymphocytes (TIL) were harvested on day 19 following B16 melanoma inoculation in WT and AireGW/+ mice treated with aCTLA-4 or isotype control antibody (iso). Representative flow cytometry plots and average cumulative frequencies of Ki67+ (A) and KLRG1+ and granzyme B+ (B) among CD4+ T cells. n = 9–12 in each group. One-way ANOVA and two-tailed t tests with P values adjusted using Hommel’s correction for multiple comparisons. *P < 0.05, **P < 0.01. (C) ELISPOT with splenocytes harvested on day 14 following B16 melanoma inoculation in WT and AireGW/+ mice treated with anti–CTLA-4 antibody (aCTLA-4) or iso. Cumulative spot forming cells (SFC)/5×105 cells secreting IL-2 with OVA and TRP-1 was analyzed. Each data point represents an individual animal. One-way ANOVA and two-tailed t tests with P values adjusted using Hommel’s correction for multiple comparisons. *P < 0.05.