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. 2017 Sep 7;2(17):e95177. doi: 10.1172/jci.insight.95177

Figure 2. REV-ERB agonist prevented the phenylephrine-induced (PE-induced) cardiomyocyte hypertrophy through transcriptional repression of the fetal gene program.

Figure 2

(A–C) Neonatal rat ventricular myocytes (NRVMs) were pretreated with vehicle or SR9009 for 24 hours and then treated with PE for 48 hours. (A) Immunofluorescent staining with α-actinin (green). Scale bar: 30 μM. (B) Quantification of cell size. *P = 0.02, n = 42–114. (C) qPCR of Nppa and Nppb. *P = 0.00009 (Nppa), *P = 0.00007 (Nppb), n = 3. (D) NRVMs were pretreated with vehicle or GSK4112 for 24 hours and then treated with PE for 48 hours. qPCR of Nppa and Nppb. *P = 0.00001 (Nppa), *P = 0.0003 (Nppb), n = 3. Statistical differences were determined by 2-tailed Student’s t test. Data are presented as mean ± SEM. Multiple comparison is corrected for by using Holm-Sidak method, with α = 0.05.