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. 2017 Sep 7;2(17):e95177. doi: 10.1172/jci.insight.95177

Figure 4. REV-ERBα regulates major gene pathways during cardiomyocyte hypertrophy in vitro.

Figure 4

RNA-Seq analysis. (A) Gene Set Enrichment Analysis (GSEA). The top pathways were shown and the complete list is in Supplemental Table 1. DCM, dilated cardiomyopathy. HCM, hypertrophic cardiomyopathy; NES, normalized enrichment score; FWER, family-wise error rate. (B) Fragments per kilobase of transcript per million mapped reads (FPKM), n = 3. Significant P values after correction for multiple comparison are indicated in the graph. Statistical differences were determined by 2-tailed Student’s t test. Data are presented as mean ± SEM. Multiple comparison is corrected for by using Holm-Sidak method, with α = 0.05. (C) ChIP-Seq gene tracks from REV-ERBα (black), MEF2a (blue), and H3K27a (red). MEF2 data is a previously published result from HL-1 cells. H3K27a is part of the ENCODE data from adult mice hearts (28, 29). (D) FPKM of Veh- and SR9009-treated hearts at baseline and 48 hours after treatment.