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. 2017 Sep 7;2(17):e91663. doi: 10.1172/jci.insight.91663

Figure 1. MBP-specific T cells activated with anti-CD3/28 fail to induce EAE.

Figure 1

Splenocytes from naive Vα2.3/Vβ8.2 TCR Tg mice were activated in vitro with anti-CD3/CD28 or myelin basic protein Ac1-11 peptide (MBP Ac1-11) for 3 days. (A) Cells were collected and adoptively transferred into B10.PL mice (10 × 106 cells per mouse). Number of mice that had clinical sign/total number of mice in each group in this representative experiment was shown as follows: anti-CD3/CD28 (0/7) and APC/Ag (9/9). (B) Supernatants were analyzed by ELISA for IFN-γ, IL-17A, and GM-CSF. Data is representative of 3 independent experiments (mean ± SEM). **P < 0.001 (Student’s t test).