Fig. 4.

Building a structural model of Mnx based on the subunit connectivity determined from native MS experiment. a Determination of the topology of Mnx complex based on observed subcomplexes released in SID. The major species observed for the released MnxEF dimers are E₁F₁, and for the released trimers are E₁F₂ (~2/3 of the trimer in the spectrum), and E₂F₁ (~1/3 of the trimer in the spectrum). The lack of a strong signal for homo-dimers and homo-trimers ruled out some possible configurations for the observed E₂F₂ and E₂F₃ subcomplexes, leaving a symmetric hexamer structure being the most reasonable solution. b With the topology proposed based on SID data, computational models of the subunits can be docked to generate possible models of Mnx complex with additional constraints from CCS and surface labeling experiments (Supplementary Methods and Supplementary Fig. 5)