Fig. 6.

Effect of BC-LI-0186 on cancer-associated MTOR mutations. a Schematic representation of cancer-associated MTOR mutations. b Leucine-dependency of cancer-associated MTOR mutations. SW620 cells were transfected with FLAG-tagged mTOR WT and the indicated mutants. The cells were starved for leucine for 1.5 and 6 h, and the cell lysates were analyzed with the indicated antibodies. c–h Effect of BC-LI-0186 on S6K phosphorylation in SW620 cells harboring mTOR WT (c), mTOR L1460P (d), mTOR I2500F (e), mTOR V2006L (f), mTOR F2108L (g), mTOR S2215Y (h). The levels of p-S6K shown in Supplementary Fig. 7a–e were quantified and displayed as graph