Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 29;7:12423. doi: 10.1038/s41598-017-12403-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Exosomes from irradiated cells activate the AKT-pathway. (a) Western blot of phospho-mTOR (Ser2448) and mTOR of cells which were incubated for 24 hours with exosomes isolated either from irradiated cells (EXO 6 Gy) or from non-irradiated cells (EXO 0 Gy). Normalization was performed to ACTIN and to cells treated with exosomes from non-irradiated cells (EXO 0 Gy). Cropped blots are displayed [n = 4; ± SD; two-sided, one-sample t-test; p-value < 0.05]. (b) Western blot of phospho-mTOR (Ser2448) and mTOR of cells which were pretreated for 1 hour with 25 µM Dynasore and incubated for 24 hours with exosomes isolated either from irradiated cells (EXO 6 Gy) or from non-irradiated cells (EXO 0 Gy). Normalization was performed to ACTIN and to cells treated with exosomes from non-irradiated cells (EXO 0 Gy). Cropped blots are displayed [n = 3; ±SD; two-sided, one-sample t-test]. (c) Western blot of phospho-S6 Ribosomal Protein (Ser240/244) and S6 Ribosomal Protein of cells which were incubated for 24 hours with exosomes isolated either from irradiated cells (EXO 6 Gy) or from non-irradiated cells (EXO 0 Gy). Normalization was performed to ACTIN and to cells treated with exosomes from non-irradiated cells (EXO 0 Gy). Cropped blots are displayed [n = 7; ±SD; two-sided, one-sample t-test; p-value < 0.05]. (d) MMP2 and MMP9 activity in the supernatants 24 hours after transfer of exosomes isolated from irradiated (EXO 6 Gy) and from non-irradiated cells (EXO 0 Gy) on BHY cells. Normalization was performed to cells treated with EXO 0 Gy. Cropped gels are displayed [n = 6; ±SD; two-sided, one-sample t-test; p-value < 0.05].